Medical Research Laboratory
In order to enhance the competiveness of Beijing Tiantan Hospital, our Core Laboratory for Clinical Medical Research (Core Lab) was established in October 2010, has been fully equipped from 2011. Our major goal of this lab is to facilitate the scientific research and preserve the valuable clinical sample specimens for Beijing Tiantan Hospital. The Core Lab became an independent department and started to operate since August, 2011. There are 9 fixed staff in the lab now, including 4 senior staff, 2 medium-grade staff, and 3 junior staff, as well as 3 employees are providing services on research designing, training on experimental skills, and bioinformatics data processing. Most of younger staff is in our team with 8 PhD and 1 Master.
Research consultations have been conducted for 409 projects from different departments of Beijing Tiantan Hospital as well as Beijing Stomatological Hospital, Friendship Hospital, Tongren Hospital, School of Traditional Chinese Medicine of Capital Medical University, Institute of Materia Medica in Chinese Academy of Medical Sciences, Shijitan Hospital, Beijing University of Chemical Technology, Peking Union Medical College Hospital, et., al. The core lab have been involved in a total of 283 projects funded by National Natural Science Foundation of China, National "Twelfth Five-Year" Plan for Science & Technology Support, Beijing Natural Science Foundation, and so on. The core lab has assisted 52 project of sample preserving, held 142 academic seminars and 11 experiment skills training curriculums since its establishment. More than 100 graduate students (Candidate for Master’s Degree or Phylosophy Doctor’s Degree) have obtained their diplomas under the assistance of the core lab since 2011.
Based on our 7 functional components including specimen library, molecular biology lab, protein lab, cell biology lab, histological lab, microscope lab, flow cytometry lab, our core lab are able to handle scientific research on gene analysis, protein , cell, and tissue level. More than 80 pieces of equipment in this lab, such as PCR instrument, Real-Time PCR instrument, Agilent 2100 bioanalyzer, nucleotide electrophoresis and imaging system, AKTA protein purify UPC100 system, wet and semi dry transfer unit, protein electrophoresis system, flow cytometry, multifunctional microplate reader, multifunctional chemiluminenscence instrument, ultraviolet-visible pectrophotometer, high content screening instrument, automatic workstation, microscope with laser capture microdissection function, confocal laser scanning microscope, fluorescence microscope, automation-tissue-dehydrating machine, paraffin embedding system, semi-automatic machine paraffin sections, Microtome-Cryostat, ABI 3730 gene analyzer, Affymetrix chip scanner, biosafety cabinet, super clean bench, ultrapure water system, ultra-low temperature freezers, constant temperature incubators, constant temperature cabnet drier, high-speed freezing centrifuge, table concentrator, ultrasonic cleaner, high-pressure steam sterilization pot, ensure the feasibility of acomplishing multiple projects. The management system and application rules were adopted according to the PDCA (Plan-Do-Check-Action) principle, and the daily operation of the core lab have satisfied all kind of demand on the scientific research in the Tiantan Hospital.
What we can perform:
1 Nucleotide-related molecular biology experiments
DNA/RNA/plasmid extraction, PCR, real-time PCR, electrophoresis, gel extraction, nucleotide concentration measurement, vector construction, DNA sequencing, chip scanning.
2 Protein-related experiments
Protein extraction and concentration measurement,
SDS-PAGE, Western Blot, co-IP, protein protein expression and purification.
3 Cell biology experiments
Primary cell separation, cell culture, tissue explant culture, cell proliferation and regulation, cell differentiation and regulation, cell senescence and apoptosis, cell signal transduction and so on.
4 Immunological experiments
ELISA, paraffin section, frozen section, hematoxylin and eosin staining, tissue array testing, immunohistochemical staining, immunofluorescence staining, in situ hybridization, laser confocal 3D imaging, laser capture microdissection
5 Flow cytometric multi-color multi-parameter analysis and sorting
Our Flow Cytometry Facility provides flow cytometric analysis and cell sorting services to all researchers in our core. We house a BD FACS Aria II high speed cell sorter. It has three lasers: a blue 488nm, a red 633/640nm, and a violet 405nm. The optical configuration allows multi-parameter detection of up to 9 fluorescent parameters and 2 light scatter parameters. The FACS Aria II is also capable of sorting through over 50 million cells per hour, with up to four populations simultaneously.
Please contact us with if you have any questions regarding the facility, flow cytometry, or the development of new applications.
6 Clinical specimen processing and preservation
Serum/plasma isolation, blood cell isolation, preservation of blood, cells tissue samples, and urine.
In order to enhance the competiveness of Beijing Tiantan Hospital, our Core Laboratory for Clinical Medical Research (Core Lab) was established in October 2010, has been fully equipped from 2011. Our major goal of this lab is to facilitate the scientific research and preserve the valuable clinical sample specimens for Beijing Tiantan Hospital. The Core Lab became an independent department and…